Table III. Comparison of mean relative gene expression in patients with primary myelofibrosis and healthy controls at baseline.
| Gene | Gene name | Patients | Controls | p-Value* | ||
|---|---|---|---|---|---|---|
|
| ||||||
| No. of samples analyzed | Mean ΔCT (95% CI) | No. of samples analyzed | Mean ΔCT (95% CI) | |||
| SPARC-BM | Secreted protein, acidic, cysteine-rich | 9 | 4.953 (3.84-6.06) | 6 | 6.207 (5.62-6.79) | 0.0659 |
| SPARC-PB | 11 | 5.852 (4.17-7.54) | 6 | 4.941 (3.93-5.96) | 0.4113 | |
| COX2-BM | Cyclo-oxygenase 2 | 9 | 7.259 (6.27-8.25) | 6 | 8.998 (8.08-9.92) | 0.0132 |
| COX2-PB | 11 | 7.509 (6.32-8.70) | 6 | 11.40 (9.74-13.1) | 0.0004 | |
| KRAS-BM | Kristen rat sarcoma viral oncogene | 9 | 11.60 (7.33-15.87) | 6 | 6.548 (4.526-8.570) | 0.0538 |
| KRAS-PB | 11 | 12.35 (8.24-16.46) | 6 | 7.974 (7.05-8.90) | 0.1064 | |
| FOS-BW | FBJ murine osteosarcoma viral oncogene homolog | 9 | 2.656 (1.952-3.359) | 6 | 2.720 (1.77-3.67) | 0.895 |
| FOS-PB | 11 | 3.349 (2.38-4.32) | 5 | 4.503 (3.53-5.48) | 0.1197 | |
| CXCR4-BM | Chemokine (C-X-C motif) receptor 4 | 9 | 5.072 (3.98-6.17) | 6 | 2.707 (2.30-3.11) | 0.0017 |
| CXCR4-PB | 11 | 4.351 (3.33-5.37) | 4 | 5.284 (4.618-5.949) | 0.2575 | |
| PAX5C-BM | Paired box 5 C-terminus | 6 | 9.539 (7.83-11.25) | 4 | 6.473 (4.80-8.15) | 0.0108 |
| PAX5C-PB | 11 | 9.971 (8.64-11.31) | 3 | 7.482 (5.83-9.13) | 0.0592 | |
| SOCS2-BM | Suppressor of cytokine signaling 2 | 8 | 11.47 (8.46-14.47) | 4 | 8.828 (7.76-9.90) | 0.1634 |
| SOCS2-PB | 11 | 13.19 (10.41-15.97) | 2 | 9.400 (6.80-12.00) | 0.7099 | |
| SOCS3-BM | Suppressor of cytokine signaling 3 | 10 | 5.384 (3.946-6.822) | 4 | 4.724 (1.71-7.74) | 0.5838 |
| SOCS3-PB | 11 | 5.011 (3.50-6.52) | 3 | 5.562 (2.15-8.97) | 0.6984 | |
| HIF1A-BM | Hypoxia inducible factor 1A | 10 | 7.303 (6.34-8.27) | 4 | 5.106 (4.20-6.01) | 0.0095 |
| HIFIA-PB | 11 | 6.259 (5.39-7.13) | 3 | 6.32 (5.90-6.74) | 0.9383 | |
*
Differences in mean expression between patient sample and control samples were compared using unpaired t-tests. p<0.05 was considered significant. CT: cycle threshold value; CI: confidence interval; BM: bone marrow; PB: peripheral blood.