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. 2016 Jun 20;148(3):304–314. doi: 10.1111/imm.12612

Figure 1.

Figure 1

Kinetics of interferon‐γ (IFN‐γ) production in 129SvEv wild‐type (WT) and interleukin‐10 (IL‐10) ‐deficient mice monoassociated with Escherichia coli NC101. (a) IFN‐γ production by unseparated mesenteric lymph node (MLN) cells from germ‐free (GF) or E. coli NC101 monoassociated IL‐10‐deficient mice, 129SvEv background. (b) IFN‐γ production by unseparated MLN cells from GF or E. coli NC101 monoassociated 129SvEv WT mice. MLN cells were stimulated in vitro with 10 μg/ml E. coli NC101 lysate. Supernatants were collected 72 hr later and IFN‐γ was quantified by ELISA. (c) IFN‐γ mRNA expression in transverse colon of GF or E. coli NC101 monoassociated 129SvEv WT mice determined by quantitative PCR. Copy number is normalized to GAPDH and expressed as fold change relative to GF mice. Each dot represents an individual mouse analysed in either two independent experiments (GF or day 4, 7, 14, 30 WT mice or GF IL‐10‐deficient mice) or one experiment (IL‐10‐deficient mice day 4, 7 and 30). Day after monoassociation is indicated on the x‐axis. Data were analysed using one‐way analysis of variance with Tukey's multiple‐comparison post‐test. P‐values indicate statistically significant differences between IFN‐γ produced by E. coli NC 101 lysate‐stimulated MLN cells in (a) and (b) or IFN‐γ mRNA expressed in transverse colon in (b) from GF mice compared with mice at 4, 7, 14 or 30 days after monoassociation with E. coli NC101. In addition, statistically significant differences in (b) 4‐day versus 7‐day P < 0·05, 4‐day versus 14‐day P < 0·05, 4‐day versus 30‐day P < 0·001; in (c), 4‐day versus 7‐day P < 0·001, 4‐day versus 14‐day P < 0·01, 4‐day versus 30‐day P < 0·001.