Table 1.
Proportion of interferon‐γ‐ or IL‐10‐positive cells in mesenteric lymph node cell subsets
| IFN‐γ positive cells | ||||
|---|---|---|---|---|
| Germ‐free | E. coli monoassociated for 4 days | |||
| Percent of subset in MLNa | Percent IFN‐γ‐positive of subsetb | Percent of subset in MLN | Percent IFN‐γ‐positive of subset | |
| CD4 | 32·8 ± 1·6 | 0·74 ± 0·06 | 31·5 ± 1·9 | 1·13 ± 0·06** |
| CD8 | 14·8 ± 0·7 | 10·68 ± 0·83 | 12·3 ± 0·7 | 11·46 ± 0·55 |
| NKp46 | 0·39 ± 0·01 | 3·55 ± 0·83 | 0·44 ± 0·01 | 12·42 ± 1·23*** |
| IL‐10 positive cells | ||||
|---|---|---|---|---|
| Germ‐free | E. coli monoassociated for 4 days | |||
| Percent of subset in MLN | Percent IL‐10 positive of subsetc | Percent of subset in MLN | Percent IL‐10 positive of subset | |
| CD4 | 32·8 ± 1·6 | 0·17 ± 0·04 | 31·5 ± 1·9 | 0·25 ± 0·04 |
| CD19 | 37·5 ± 2·0 | 1·40 ± 0·05 | 43·5 ± 1·7 | 1·43 ± 0·07 |
Values represent mean ± SEM of cells expressing the indicated cell surface marker evaluated after overnight stimulation with E. coli NC101 lysate followed by 5 hr incubation with ionomycin plus PMA, with Golgi stop during the last 4 hr. MLN from E. coli NC101 monoassociated mice n = 4; MLN from GF mice n = 6.
Values represent mean ± SEM of the proportion of IFN‐γ positive cells determined after gating on each subset.
Values represent mean ± SEM of the proportion of IL‐10 positive cells determined after gating on each subset.
**P < 0·01 compared to dual CD4 positive – IFN‐γ positive cells in MLN of GF mice; ***P < 0·001 compared to dual NKp46 positive – IFN‐γ positive cells in MLN of GF mice. Otherwise differences between percent of cytokine positive cells of each subset from E. coli monoassociated and GF mice are not statistically significant.