Table 2. Strains and plasmids used in this study.
| Strains and plasmids | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| C. acetobutylicum DSM 1731 | Wild type strain | DSMZ |
| C. acetobutylicum DSM 1731 adhE1::int(807) | The adhE1 II intron insertion gene mutant of C. acetobutylicum DSM 1731 | This study |
| C. acetobutylicum DSM 1731 adhE2::int(846) | The adhE2 II intron insertion gene mutant of C. acetobutylicum DSM 1731 | This study |
| C. acetobutylicum DSM 1731 bdhA::int(133) | The bdhA II intron insertion gene mutant of C. acetobutylicum DSM 1731 | This study |
| C. acetobutylicum DSM 1731 bdhB::int(669) | The bdhB II intron insertion gene mutant of C. acetobutylicum DSM 1731 | This study |
| C. acetobutylicum DSM 1731 yqhD::int(405) | The yqhD II intron insertion gene mutant of C. acetobutylicum DSM 1731 | This study |
| C. acetobutylicum DSM 1731 SMB_P058::int(150) | The SMB_P058 II intron insertion gene mutant of C. acetobutylicum DSM 1731 | This study |
| E. coli JM109 | recA1 mcrB + hsdR17 | Lab storage |
| E. coli TOP10 | mcrA Δ(mrr-hsdRMS-mcrBC) recA1 Used for plasmid methylation before transformed into C. acetobutylicum | Invitrogen |
| Plasmids | ||
| pAN2 | Ф3tI, p15aori, TetR | 16 |
| pMTL008 | Deprived from pMTL007-cac824I71a, replacement of the lacI-traJ-oriT-fac promoter fragment by Pthl | 17 |
| pMTL008-adhE1 | adhE1 gene II intron insertion inactivation plasmid | This study |
| pMTL008-adhE2 | adhE2 gene II intron insertion inactivation plasmid | This study |
| pMTL008-bdhA | bdhA gene II intron insertion inactivation plasmid | This study |
| pMTL008-bdhB | bdhB gene II intron insertion inactivation plasmid | This study |
| pMTL008-yqhD | yqhD gene II intron insertion inactivation plasmid | This study |
| pMTL008-SMB_P058 | SMB_P058 gene II intron insertion inactivation plasmid | This study |
Abbreviations: TetR tetracycline resistance, Ф3tI, Ф3TI methyltransferase gene of Bacillus subtilis phage Ф3TI; Pthl, the promoter of thiolase gene from C. acetobutylicum; DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.