Table 2. Sample and analysis overview.
Sampling date | Stations sampled | Spatial replicates | Temp. monitored* | Abiotic soil properties** | Functl. profiling (Biolog) | 16S amplicon seq.*** | ITS amp. seq.*** | 18S amp. seq*** |
---|---|---|---|---|---|---|---|---|
2011–08–31 | All | None | No | Yes | Yes | No | No | No |
2012–08–30 | All | 4 × 1700 m, 4 × 2400 m | No | Yes | Yes | Yes | Yes (−2) | Yes (−4) |
2013–08–19 | 1700 m | None | Yes† | No | No | Yes | No | Yes |
2013–08–25 | All | 4 × 1700 m, 4 × 2400 m | Yes† | Yes | Yes e.r. | Yes | Yes | Yes |
2013–09–06 | 1700 m | None | Yes† | No | No | Yes | No | Yes |
2013–10–27 | 1700 m, 2400 m | 2 × 4 | Yes† | Yes | Yes | Yese.r. | No | Yese.r. |
2014–05–08 | 2400 m | None | Yes | No | No | Yes | No | No |
2014–09–10 | All | 15 × 1700 m, 15 × 2400 m | Yes | Yes | Yes e.r. | Yese.r. | No | No |
*Soil temperature data available from on-site sensors previous to sampling.
**Soil texture (sand, silt and clay), temperature during sampling, humidity, pH, organic matter (%), soil organic matter (SOM), total nitrogen (%).
***Number of samples or replicates for which sequencing failed indicated in parentheses if applicable.
†During 2012–2013 temperatures were not measured at the 1500, 1600 or 2000 m stations due to equipment failure.
e.r. Excluding replicates. 16S amplicon sequencing was however carried out for 7 replicates from 2014 taken at 2400 m.