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. 2016 Jun 20;6:28257. doi: 10.1038/srep28257

Table 2. Sample and analysis overview.

Sampling date Stations sampled Spatial replicates Temp. monitored* Abiotic soil properties** Functl. profiling (Biolog) 16S amplicon seq.*** ITS amp. seq.*** 18S amp. seq***
2011–08–31 All None No Yes Yes No No No
2012–08–30 All 4 × 1700 m, 4 × 2400 m No Yes Yes Yes Yes (−2) Yes (−4)
2013–08–19 1700 m None Yes No No Yes No Yes
2013–08–25 All 4 × 1700 m, 4 × 2400 m Yes Yes Yes e.r. Yes Yes Yes
2013–09–06 1700 m None Yes No No Yes No Yes
2013–10–27 1700 m, 2400 m 2 × 4 Yes Yes Yes Yese.r. No Yese.r.
2014–05–08 2400 m None Yes No No Yes No No
2014–09–10 All 15 × 1700 m, 15 × 2400 m Yes Yes Yes e.r. Yese.r. No No

*Soil temperature data available from on-site sensors previous to sampling.

**Soil texture (sand, silt and clay), temperature during sampling, humidity, pH, organic matter (%), soil organic matter (SOM), total nitrogen (%).

***Number of samples or replicates for which sequencing failed indicated in parentheses if applicable.

During 2012–2013 temperatures were not measured at the 1500, 1600 or 2000 m stations due to equipment failure.

e.r. Excluding replicates. 16S amplicon sequencing was however carried out for 7 replicates from 2014 taken at 2400 m.