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. 2016 Jun 20;7:102. doi: 10.3389/fpsyt.2016.00102

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Copyright © 2016 Rowson, Harrell, Bekhbat, Gangavelli, Wu, Kelly, Reddy and Neigh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology of IBA-1-stained microglia was assessed using the AnalyzeSkeleton function in ImageJ. Microglia in female HIV-1 transgenic rats exhibited enhanced ramification characterized by an increased number of branches (A), junctions (B), and maximum branch length (C). Stress did not impact microglial morphology, and the average branch length was unchanged in any group (D). Data are presented as mean ± SEM. *Denotes a main effect of HIV-1 transgene. # denotes significant effect of stress in an a priori t-test. α = 0.05.