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. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: J Biomech. 2016 Feb 18;49(7):1214–1220. doi: 10.1016/j.jbiomech.2016.02.029

Figure 4. TNF-α but not mechanical stretch induced F-actin changes in human AF cells.

Figure 4

Immunocytochemistry for F-actin and vinculin in human AF cells seeded at 100,000 cells/mL immediately after 24 hours of stretch with and without TNF-α. F-actin and vinculin staining exhibited no apparent differences in cytoskeletal organization across all strain magnitudes. TNF-α treatment increased F-actin staining intensity but there were no discernable differences between strains in cytoskeletal structure with TNF- α treatment. (Magnification = 20X, Scale = 100 µm).