TABLE 2.
Antibodies
| Epitope | Function | Antibody clone | Dilution | Origina |
|---|---|---|---|---|
| Apolipoprotein A-I | HDL particles | 1C5b | 1:5000 | Monosan |
| Apolipoprotein B-100 | LDL particles | MB47c | 1:10000 | (18, 37) |
| apoE | HDL, VLDL, IDL, or LDL particles | WU E-4b | 1:500 | Santa Cruz Biotechnology |
| HNE | Oxidized lipid | HNEd | 1:300 | (18) |
| Adipophilin | Marker of intracellular lipid accumulation | AP125b | 1:10 | R&D Systems |
| ATP-binding cassette A1 | Removal of intracellular lipids | NB400-105e | 1:100 | Novus Biologicals |
| CD68 | Macrophages | EBM11b | 1:100 | Dako |
| CD163 | Macrophages | 5C6-FATb | 1:100 | Novus Biologicals |
| αSMA | α-SMC actin | 1A4c | 1:100 | Dako |
aMonosan, Uden, The Netherlands; Santa Cruz Biotechnology, Dallas, TX; R&D Systems, Minneapolis, MN; Novus Biologicals, Littleton, CO; Dako, Glostrup, Denmark.
bMouse monoclonal IgG1.
cMouse monoclonal IgG2a.
dGuinea pig polyclonal.
eRabbit polyclonal.