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. Author manuscript; available in PMC: 2016 Jun 20.
Published in final edited form as: Eur J Immunol. 2015 Feb 26;45(5):1313–1325. doi: 10.1002/eji.201445319

Figure 5. Ly-6D expression and CpG treatment induce transcriptional profile changes.

Figure 5

A) Principle component analysis from microarray of Ly-6D+ and Ly-6D− CLPs from CpG and PBS treated mice. B) Venn diagram of significant genes from 2-way comparison of microarray data sets. False discovery rate was set to 20%. C) Results of K means clustering using k=10 of differentially expressed genes with a false discovery rate of 20%. Average row normalized gene expression for each population in each cluster is presented. (D, E) Ly-6D− CLPs from three PBS and three CpG treated mice were analysed by qRT-PCR. D) HOXa9 and its downstream targets. E) E2A and its unique target FOXO1. Data show mean + SEM from 3 individual samples. Expression of the gene relative to β-actin is shown. * = p<0.05, ** = p<0.005, Student’s t-test