Table 1.
Detection of blaCTX-M ESBLs in Wetlands and Wastewater Treatment Plants by Conventional PCR
| Site | Group 1 | Group 2 | Groups 8/25a | Group 9 |
|---|---|---|---|---|
| TRE (wet) | + | − | − | − |
| TRE (dry) | − | − | − | − |
| FAM (wet) | − | − | − | − |
| FAM (dry) | − | − | − | − |
| SBI WWTPb | + | + | − | − |
| PL WWTPb | + | + | − | − |
Positive PCR reactions were confirmed by cloning and sequencing.
a
The primers used cannot distinguish group 8 from group 25.
b
If any fraction from the wastewater treatment process was positive, the site is reported as positive.
ESBLs, extended-spectrum β-lactamases; FAM, Famosa Slough; PCR, polymerase chain reaction; PL WWTP, Point Loma Wastewater Treatment Plant; SBI WWTP, South Bay International Wastewater Treatment Plant; TRE, Tijuana River Estuary; wet/dry, wet season or dry season wetland samples.