Table 3.
Summary of blaCTX-M Group 1 Clone Libraries from Wetlands and WWTPs
| Environment | Lib sizea | Unique (nt)b | Unique (aa)b | Novel (nt)c | Novel (aa)c |
|---|---|---|---|---|---|
| TRE (wet) | 80 | 61 | 45 | 56 | 41 |
| SBI WWTPd | 84 | 55 | 36 | 49 | 30 |
| PL WWTPe | 73 | 63 | 38 | 57 | 32 |
| TOTALf | 237 | 179 | 119 | 164 | 103 |
| Combinedg | 165 | 108 | 157 | 99 |
a
Total number of cloned environmental blaCTX-M group 1 DNA sequences analyzed from each library.
b
Nonredundant within a given library at either the nucleotide (nt) or amino acid (aa) level.
c
Nonredundant in the GenBank database at either the (nt) or (aa) level.
d
All positive PCR reactions from the SBI WWTP were pooled to create a single library.
e
Only the effluent of the PL WWTP was PCR positive.
f
Sum of sequences across all three libraries.
g
TOTAL less any clones that were observed in multiple libraries.
Lib, Library.