Table 2.

Potency and Maximum Response (E_Max) of Carbachol-Induced Pulmonary Arterial Contraction in the Presence and Absence of Atropine (Nonselective), or AFDX 116 (M2 at 100 nM or M1 and M3 at 1 μM Selective), 4-DAMP or DAU 5884 (M3 Selective), or Pirenzepine (M1 Selective) Muscarinic Receptor Antagonists

	AN			AH			FN
Denuded vessels Animal group	Potency	EMax	N	Potency	EMax	N	Potency	EMax	N
CCh	−5.85 ± 0.16 M	63% ± 4%	49	−5.72 ± 0.2 M	40% ± 3%	23	−5.64 ± 0.35 M	49% ± 7%	17
CCh + 1 μM Atropine	−3.10 ± 0.56 M	ND	19	−5.72 ± 0.2 M	ND	19	NA	NA
CCh + 100 nM AFDX 116	−5.70 ± 0.23 M	43% ± 3%a	21	−5.36 ± 0.22 M	34% ± 3%	12	−4.56 ± 0.31 Ma	16% ± 3%a	8
CCh + 1 μM AFDX 116	−5.04 ± 0.19 Ma	55% ± 5%	25	−5.02 ± 0.27 M	42% ± 5%	17	−3.92 ± 0.42 Ma	36% ± 8%a	12
CCh + 10 μM 4-DAMP	−3.73 ± 0.16 Ma	109% ± 15%	16	−4.14 ± 0.42 M	31% ± 8%	13	−6.33 ± 0.54 M	62% ± 10%	6
CCh + 100 nM DAU5884	−4.94 ± 0.22 Ma	47% ± 5%	21	−4.56 ± 0.35 M	50% ± 8%	8	−5.39 ± 0.42 M	54% ± 6%	12
CCh + 100 nM Pirenzepine	−5.38 ± 0.36 M	62% ± 9%	16	−5.88 ± 0.38 M	42% ± 5%	14	−5.92 ± 0.52 M	52% ± 7%	14

^aIndicates statistical significance, p < 0.05 relative to control by a one-way analysis of variance with a Bonferroni's Multiple Comparison Test. Maximum response is relative to the %T_Kmax. N is the number of arteries used for the studies.

NA, not applicable.