FIG. 4.

Histological analysis of sheep lumbar vertebrae. (A) Representative images of Von Kossa–McNeal's Tetrachrome staining of sheep lumbar vertebral bodies were taken at peri-implant sites treated with control, rhNELL-1 (0.9 mg) and rhNELL-1 (2.25 mg). (B–F) Histomorphometric quantification using Von Kossa–McNeal's Tetrachrome stained slides of samples treated with control, rhNELL-1 (0.9 mg) and rhNELL-1 (2.25 mg)-based vertebral body implantation. Bone measurements include (B) Cortical Width (Ct. Wi), (C) Percent Trabecular Bone (% Tb. Ar), (D) Trabecular Width (Tb. Wi), (E) Trabecular Number (Tb. N), and (F) Trabecular Bone Spacing (Tb. Sp). n = 3 lumbar vertebrae per dose rhNELL-1, n = 6 control-treated lumbar vertebrae. n = 6 sheep in total. *p < 0.05 and **p < 0.01 in comparison to control. Scale bar: 100 um. Color images available online at www.liebertpub.com/tea