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. 2016 Jun 6;48(6):536–543. doi: 10.1093/abbs/gmw031

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© The Author 2016. Published by Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

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Figure 1. — Schematic diagram and purification of Physarum myosin hybrid constructs (A) Schematic diagram of Physarum myosin hybrid constructs. (B) SDS-PAGE (12.5%) of purified Physarum myosin hybrid constructs associated with LCs of Physarum and/or scallop myosins. Lane M, molecular weight marker; lane 1, Ph·HC/PhRLC/PhELC; lane 2, Ph·HC/ScRLC/ScELC; lane 3, Ph·HC/ScRLC/PhELC; lane 4, Ph·HC/PhRLC/ScELC. (C) SDS-PAGE (12.5%) of purified myosin mutant Ph·HC/PhRLC/PhELC-3A and hybrid myosin mutant Ph·HC/ScRLC/PhELC-3A. Lane M, molecular weight marker; lane 5, Ph·HC/PhRLC/PhELC-3A; lane 6, Ph·HC/ScRLC/PhELC-3A. (D) Glycerol PAGE showing LCs of expressed Physarum hybrid myosin Ph·HC/PhRLC/ScELC (a), expressed Physarum hybrid myosin Ph·HC/ScRLC/ScELC (b), Physarum myosin (c), and purified scallop myosin (d). (E) Glycerol PAGE showing LCs of expressed Physarum myosin mutants of Ph·HC/PhRLC/PhELC-3A (e) and hybrid myosin mutant Ph·HC/ScRLC/PhELC-3A (f).