Table 1.
Physarum myosin constructs | The relative actin-activated ATPase activities | ||
---|---|---|---|
EGTA (%)a | Ca2+ (%)b | Effect of Ca2+ (%)c | |
Ph·HC/PhRLC/PhELC | 100 | 3.33 | 96.6↓↓↓ |
Ph·HC/ScRLC/PhELC | 100 | 20.10 | 79.90↓↓ |
Ph·HC/PhRLC/ScELC | 100 | 60.19 | 39.81↓ |
Ph·HC/ScRLC/ScELC | 100 | 101.74 | 1.74 |
Ph·HC/PhRLC/PhELC-3A | 100 | 93.13 | 6.87 |
Ph·HC/ScRLC/PhELC-3A | 100 | 97.51 | 2.49 |
aThe actin-activated ATPase activities of Physarum myosin constructs were considered as 100% in EGTA.
bThe actin-activated ATPase activities in Ca2+ / in EGTA × 100.
cEffect of Ca2+ (%)c = ∣Ca2+ (%)b − EGTA (%)a∣/EGTA (%)a × 100; ↓means inhibition; means little activation and no significant difference; means little inhibition and no significant difference.