Table 3.

Commercially available immunologic tests for antibodies against Neospora hughesi

Test	Laboratory	Interpretation	Reported performance
IFAT100	UC Davis	Serum positive at ≥1:320; negative at <1:40 CSF positive at ≥1:5	Serum Se 100%, Sp 100% at cutoff of 1:640 Serum Se 100%, Sp 71% at cutoff of 1:320 Se and Sp estimates calculated using samples from experimentally infected horses, not EPM cases
ELISA62	EDS	Serum positive at ≥1:500 CSF positive at ≥1:5 Serum:CSF titer ratio provides most accurate EPM diagnosis	Serum Se 94%, Sp 95% compared to WB detection antibodies (not EPM cases)

IFAT, indirect fluorescent antibody test; ELISA, enzyme‐linked immunosorbent assay; UC Davis, University of California at Davis; EDS, Equine Diagnostic Solutions (Lexington, KY); CSF, cerebrospinal fluid; EPM, equine protozoal myeloencephalitis; Se, sensitivity; Sp, specificity; WB, Western blot.