Fig. 7.

IP6K1 modulates the invasive potential of cancer cells. (A) HCT116 cells stably expressing NT or shIP6K1–1 were allowed to invade a matrigel matrix to move towards a high-serum gradient for 24 h. Representative images show cells that migrated through the gel to the other side of the membrane, visualized by staining with DAPI. Scale bars represent 50 μm. (B) Quantification of (A); bar graphs show the number of invaded cells normalized to the NT control. Data are mean ± SEM from five independent experiments, and were analyzed by a one sample t-test. (C) Immunoblot analysis of epithelial marker E-Cadherin in HCT116 cells expressing NT or shIP6K1–1. (D) Quantification of (C); levels of the epithelial marker E-cadherin are indicated as a ratio with respect to the levels of GAPDH which was the loading control. Data represents mean ± SEM from three independent experiments and was analyzed using a two tailed unpaired Student's t-test. **P ≤ 0.01; ***P ≤ 0.001.