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. Author manuscript; available in PMC: 2016 Jun 20.
Published in final edited form as: Eur Cell Mater. 2011 Sep 20;22:168–177. doi: 10.22203/ecm.v022a13

Figure 3.

Figure 3

Regulation of matrix accumulation by ligand-modified agarose over 8 days of culture in basal or chondrogenic medium (10 ng/mL TGF-β1 and 100 nM dexamethasone). (a) Proteoglycan synthesis as measured by incorporation of 35S-sulfate over the final 24 hoursof culture (Day 7–8) for unmodified and functionalized agarose with 100 μM RGE, 100 μM RGD, 1 μM FnIII7-10, or 15 μM GFOGER final ligand densities. (b) Total sGAG accumulation in the gels after the 8-day culture period as measured by the DMMB assay. (C) Protein synthesis as measured by incorporation of 3H-proline over the final 24 hours of culture; and (d) total collagen accumulation as measured by assaying for hydroxyproline (HP) content. Data represent the mean ± SEM, n = 4–6 gels/condition. Significance vs. chondrogenic for a given gel is indicated by * (p<0.0001) or + (p<0.001).