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. 2016 Mar 31;7:100–111. doi: 10.1016/j.ebiom.2016.03.037

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. S1. — Characterization of adipocyte phenotypes following differentiation of Bmal1-dLuc fibroblasts. (A) Representative microscopic image illustrating the cytoplasmic accumulation of lipids in Bmal1-dLuc cells stained with Oil Red O staining after differentiation using the methods of Huo et al., 2010, Huo et al., 2012. (B) Real-time PCR determinations (mean ± SEM) of PPARγ and adiponectin mRNA expression in Bmal1-dLuc fibroblast and differentiated adipocyte cultures. PPARγ (left panel) and adiponectin (right panel) mRNA signal were normalized to b-actin mRNA levels in each sample and the fold difference was determined by adjusting these values in relation to the average of undifferentiated fibroblast cultures, which was set at 1. The relative levels of PPARγ and adiponectin mRNA in differentiated adipocyte cultures were increased by 8-fold and 450-fold, respectively and were significantly greater (p < 0.05) than that found in undifferentiated Bmal1-dLuc fibroblasts.