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. 2016 Mar 31;7:100–111. doi: 10.1016/j.ebiom.2016.03.037

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. S2. — Effects of palmitate (PAL) and AICAR treatment on phospho-AMPK activity in Bmal1-dLuc fibroblasts. Using an ELISA assay for phospho-AMPKα (Invitrogen), p-AMPK activity was analyzed in cultures treated with AICAR (500 μM) in conjunction with palmitate (250 μM) administration. Effects of treatment with PAL (VEH + PAL) or AICAR (AICAR + BSA) alone on p-AMPK activity were also tested. Control cultures were treated with vehicle (DMSO) and BSA (VEH + BSA). Plotted values denote determinations of colorimetric signal intensity (mean ± SEM) in each treatment group (n = 4) that were normalized to the average signal for controls, which was arbitrarily set at 100%. Phospho-AMPK activity was significantly decreased (p < 0.05) in Bmal1-dLuc fibroblasts treated with PAL (VEH + PAL) and was significantly increased (p < 0.05) in cultures treated with AICAR alone (AICAR + BSA) relative to that found in controls (VEH + BSA).