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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Cell Biochem. 2016 Feb;117(2):439–447. doi: 10.1002/jcb.25290

Fig. 3.

Fig. 3

HuR and miR-633 competition. A: Mouse C3H10T1/2 mesenchymal cells were co-transfected with a reporter gene driven by the mouse BMP2 promoter (nt. −1,237 to 471 relative to the distal promoter, Bmp2Luc) alone or with nt. 85−449 from the mouse 3′UTR inserted downstream of luciferase (Bmp2Luc-mUCS). These cells were co-transfected with either a GFP-expressing control plasmid or a plasmid expressing HuR [Fan and Steitz, 1998]. The average luciferase reporter activity is shown ±SEM, n =12, Bmp2LucUCS with GFP vs. with HuR P <0.02. B: C3H10T1/2 cells were co-transfected with either Bmp2Luc-mUCS or RPL10Luc-3′UTR with the GFP or HuR expression vectors and with the Negative Control (NC) or miR633 (633) miRNA precursors as indicated (n =3). Although, HuR activated both the Bmp2 driven and the RPL10-driven vectors bearing the UCS or the 3′UTR respectively (P <0.003, n =3 each), HuR failed to activate in the presence of miR-633.