. Author manuscript; available in PMC: 2016 Jul 1.

Published in final edited form as: Nat Biotechnol. 2015 Dec 7;34(1):78–83. doi: 10.1038/nbt.3439

Table 1. Recombinase Mediated Cassette Exchange to insert CRISPR^h alleles at their target locus.

Target Gene	Injected Eggs	G₀ Crossed	% Founders	% cassette exchange progeny	Crosses of fertile G₁ containing CRISPR^h allele
					G1 cross	G₂ progeny		transmission rate
					G1 cross	CRISPR^h +	CRISPR^h -	transmission rate
AGAP007280	540	56	≥7.1% (4/56)^*	0.38% (15/4000)	1 ♀G1 x wt	34	2	94.4%
AGAP007280	540	56	≥7.1% (4/56)^*	0.38% (15/4000)	8 ♂G1 x wt	666	3	99.6%
AGAP011377	500	21	≥4.8% (1/21)^*	0.13% (4/2990)	1 ♀G1 x wt	35	0	100.0%
AGAP005958	400	49	≥2.0% (1/49)^*	0.05% (2/4000)	1 ♂G1 x wt	236	0	100.0%

CRISPR homing constructs were injected into the respective docking lines with a plasmid source of vasa-driven integrase. Successful recombinase-mediated exchange events were scored visually for the replacement of GFP at the docking site with the RFP contained within the CRISPR^h construct. The proportion of G₁ progeny containing putative cassette exchange events is also shown.

In these cases the progeny were screened from group crosses hence the estimate for the number of founders is a minimum.

Table 1. Recombinase Mediated Cassette Exchange to insert CRISPRh alleles at their target locus.

Table 1. Recombinase Mediated Cassette Exchange to insert CRISPR^h alleles at their target locus.