Table 2. CRISPRh homing rates remain high across several generations.
| Line | % of progeny with CRISPRh allele in crosses to wt |
Average Transmission Rate per generation | Average Homing Rate Per Generation | |||
|---|---|---|---|---|---|---|
| G2 cross | G3 cross | G4 cross | G5 cross | |||
| AGAP011377 ♂+/- | 91.4% (581/636) | 88.4% (1442/1631) | 93.7% (1550/1654) | 97.3% (491/505) | 92.37% | 85% |
| AGAP011377♀+/- | 91.7% (55/60) | 76.1% (70/92) | 85.2% (121/142) | 84.56% | 69% | |
| AGAP005958♂+/- | 97.9% (1654/1689) | 96.4% (268/278) | 97.17% | 94% | ||
| AGAP005958♀+/- | - | - | - | - | ||
| AGAP007280♂+/- | 99.6% (1377/1383) | 98.8% (499/505) | 99.19% | 98% | ||
| AGAP007280♀+/- | 99.2% (255/257) | 99.22% | 98% | |||
Each generation heterozygous individuals of each sex from each homing line were crossed to wild type mosquitoes and the frequency of the CRISPRh allele among the progeny estimated by scoring visually for the presence of the RFP gene contained within the CRISPRh construct. In all cases the progeny of the CRISPRh male cross were used to maintain the line each generation. Homing rate is calculated as the percentage of wild type chromosomes converted to homed chromosomes (i.e (transmission rate-0.5)*2)