Figure 2.

Results of the microcosm experiment transplanting strain QLW-P1DMWA-1 (QLW, representing lineage F10) in water from Lake Mondsee screened through a 100 μm sieve for removal of metazooplankton. This treatment was run in triplicates (blue triangles, average and s.d.). For comparison, the strain was also inoculated in sterile water from Lake Mondsee (black circles) and sterile water from Pond-1 (red triangles) but with different volumes and cell numbers. The treatments with 100 μm-screened and sterile Lake Mondsee water received the same inoculum volumes, but extraction of the 0 h sample failed in the latter treatment. In all, 100 μm-screened water of Lake Mondsee not inoculated with strain QLW-P1DMWA-1 served as negative control (green squares). In all treatments, 50% of medium was replaced each third day (gray arrows). Sampling on day 3 took place immediately before replacement of medium. The abundance of strain QLW-P1DMWA-1 was determined by quantitative PCR.