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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Atherosclerosis. 2016 Feb 13;247:207–217. doi: 10.1016/j.atherosclerosis.2016.01.043

Figure 5.

Figure 5

Inhibition of autophagy in endothelial cells from non-diabetics impairs endothelial function. A: Insulin-induced activation of eNOS by phosphorylation at Ser1177 (red channel) was measured in endothelial cells from non-diabetics and diabetics. Also shown are vWF (green channel), DAPI (blue channel) and merged images (upper left).Cells from diabetic subjects displayed impaired activation (#P<0.01). B: Treatment of endothelial cells isolated from non-diabetic subjects (n = 6) with 10 nM bafilomycin for 60 minutes increased p62 levels, indicating impaired autophagy (#P≤0.01). C: Bafilomycin impaired insulin-induced eNOS activation (red channel) in endothelial cells from non-diabetics (*P<0.05). Also shown are vWF (green channel), DAPI (blue channel) and merged images (upper left).