Fig 2. 5-bromo-3-indolyl-β-D-galactopyranoside staining detected a decrease in viral titer of adenovirus after nitrogen gas plasma treatment.

A cell culture medium of adenovirus vector (AxCAiLacZ)-infected HEK293 cells (1.0 x 10⁹ PFU/ml) was air-dried onto a glass coverslip and treated with nitrogen gas plasma using the BLP-TES device at 1.5 kpps for 0, 5, and 15 min. After the treatment, adenovirus was recovered from the spots and subjected to 5-bromo-3-indolyl-β-D-galactopyranoside staining as described in Materials and Methods.