Fig 3. Infectious titer assay using antibody against adenovirus hexon protein show decreased viral infectivity of adenovirus after nitrogen gas plasma treatment.

A cell culture medium of adenovirus vector (AxCAwt2)-infected HEK293 cells (1.0 x 10⁹ PFU/ml) was air-dried onto a glass coverslip and treated with nitrogen gas plasma using a BLP-TES device at 1.5 kpps for 0, 5, and 15 min. After treatment, adenovirus was recovered from the spots and then subjected to an infectious titer assay using antibody against adenovirus hexon protein to determine the number of infected cells.