Fig 4. Nitrogen gas plasma treatment induces damage to the genomic DNA of adenovirus.

A cell culture medium of adenovirus vector (AxCAiLacZ)-infected HEK293 cells (1.0 x 10⁹ PFU/ml) was air-dried onto a coverslip and treated with nitrogen gas plasma using the BLP-TES device at 1.5 kpps for 0, 5 and 15 min. The recovered samples were then subjected to polymerase chain reaction (PCR) analysis using primers designed to amplify the hexon gene of adenovirus. A PCR product of the anticipated size was observed at 0 min (indicated by arrowhead), but the intensity of the band progressively diminished after gas plasma treatment for 5 and 15 min. The molecular marker lane (M) is 100 kb DNA ladder. Numbers shown on the left correspond to the molecular size (bp).