Fig 6. Nitrogen gas plasma treatment has no effect on adenovirus hexon protein.

(A) Nitrogen gas plasma-treated cell culture medium of HEK293 cells infected with adenovirus vector (AxCAiLacZ) (1.0 x 10⁹ PFU/ml) was subjected to immunochromatography using the Quick Navi-Adeno kit (Denka Seiken Co., Ltd., Tokyo, Japan). The bands corresponding to adenovirus hexon protein are indicated by an arrowhead. C: Control line; T: Test line. (B) The band intensities of test lines of the immunochromatography in three independent experiments were compared using Image J software. The band intensity of the test line for the 0 min sample in each experiment was taken as 100%. Band intensities of test lines of adenovirus samples treated for 0, 5 and 15 min are shown. Immunochromatography showed no significant change in the hexon protein of adenovirus after nitrogen gas plasma treatment for up to 15 min.