Table 1.

Simulation parameters. All parameters were estimated from the bead-based experiments 12 except Keq, which was adjusted so that the simulation results matched the experimental results. The experimentally measured velocity, diffusion constant (from the linear term of the mean square displacement fit), and quadratic term of the mean square displacement fit are listed in the Parameter column under the Measured parameters heading. The measured parameters for the simulations are obtained from fits to the 1-D simulations in Figure 2 See main text for descriptions of the simulation parameters.

Parameter	Simulation 1	Simulation 2
u(0,x) (μM)	10	7
Da (μm2 s−1)	0.05	0.05
k (s−1)	0.01667	0.01667
Db (μm2 s−1)	0.02	0.02
bc (μM)	10	2
bn	4800	960
Keq (μM)	40	0.3
Measured parameters
Velocity 0.10 ± 0.02 μm s−1	0.089 ± 0.005 μm s−1	0.09 ± 0.01 μm s−1
Db 0.03 ± 0.02 μm s−1	0.026 ± 0.001 μm2 s−1	0.030 ± 0.001 μm2 s−1
Quadratic MSD term 0.012 ± 0.003 μm2 s−2	0.016 ± 0.002 μm2 s−2	0.019 ± 0.001 μm2 s−2