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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Am Chem Soc. 2016 Jun 3;138(23):7411–7421. doi: 10.1021/jacs.6b03341

Figure 3.

Figure 3

Optical absorbance spectra and product analysis in the reaction of 560 μM NH(OH)-CAM with 560 μM anaerobic CmlIox (red spectrum), which yields Sample B (black spectrum) which contains NO-CAM product (black HPLC trace). Addition of O2 yields Sample C (blue spectrum) which contains primarily CmlIox and CAM product (blue HPLC trace). Buffer: 50 mM Bicine, pH 9, 4 °C.