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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Am Chem Soc. 2016 Jun 3;138(23):7411–7421. doi: 10.1021/jacs.6b03341

Figure 7.

Figure 7

Single turnover reaction of P with 0.5 equiv NH2-CAM (red trace) and 1.0 equiv NH(OH)-CAM (blue trace). Single wavelength (500 nm) stopped flow traces for the reaction of 260 μM P and 130 μM NH2-CAM or 260 µM NH(OH)-CAM (post-mix concentrations) in the presence of excess O2. No P reformation is observed in the reaction with the native substrate NH2-CAM, suggesting that the in-vitro reaction ends in the diferric state. The reactions using the same concentrations of NH2-CAM and NH(OH)-CAM give the same results but the reformation of P in the case of the latter substrate is masked due to the rates of the decay and re-formation reactions. Buffer: 50 mM Bicine, pH 9, 4 °C.