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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Am Chem Soc. 2016 Jun 3;138(23):7411–7421. doi: 10.1021/jacs.6b03341

Table 1.

Oxygen Incorporation into CAM Products in the Reaction of P with NH2-CAMa

oxygen isotope: atmosphere / P / solvent
product oxygen
incorporation		 18O2/18O2 /H216O
%		 16O2/18O2/H216O
%		 16O2/16O2 /H218O
%
CAM 16O, 16O
16O, 18O
18O 18O		 0.4 ± 0.2
7 ±
2 93 ± 2		 10 ± 1
44 ± 1
47 ± 2		 100
0
0
NO-CAM 16O
18O		 4 ± 1
96 ± 1		 8 ± 6
92 ± 6		 98 ± 2
3 ± 2
a

16O2-P or 18O2-P was reacted with 0.3 equivalents NH2-CAM under different atmosphere and solvent conditions shown. Resultant products were analyzed by LCMS as described in the Experimental Procedures. All reactions done in buffer: 50 mM Bicine, pH 9, 4 °C.