Skip to main content
. 2016 Mar 4;44(11):5105–5122. doi: 10.1093/nar/gkw113

Figure 5.

Figure 5.

Knockdown (KD) of pancNusap1 mimicked irreversible differentiation of PC12 cells induced by cAMP (A) The effect of pancNusap1 KD on the expression level of Nusap1 in Ndiff cells. Gapdh was used as a control. Empty vector-introduced PC12 cells were used as a negative control. (B) Histone modification status of the Nusap1 promoter in pancNusap1-KD PC12 cells under reversible differentiation condition. The expression of sh-pancNusap1 was induced by Dox at day 5. Empty vector-infected PC12 cells were used as a control. (C) The effects of pancNusap1 KD on cell proliferation in Ndiff cells. The numbers of living cells were counted. (D) Proportion of the EdU+ and the Ki67+ cells in pancNusap1-KD PC12 cells after NGF deprivation. After 7 days of differentiation, NGF was removed to allow proliferation. White arrowheads indicate locations of proliferating infected cells (triple-positive for Ki67, EdU and GFP). In (A–C), viral vectors were introduced at day 3. In (A–D), values are mean ± SD (n = 3). Statistical analysis was performed using Student's two-tailed t-tests, or the Tukey-Kramer multiple comparison test. N.S. = not significant. *P < 0.05; **P < 0.01; ***P < 0.001.