Figure 2.

Validation of E-MAP results showing suppression of rad55 phosphorylation site mutants by nam7Δ using an independent strain background. (A) Plate assay measuring the sensitivity of W303 strains with the indicated genotypes to MMS. Serial dilutions (1:5) of cultures were spotted onto YPD containing 0.015% MMS and grown at 30°C for 2 days prior to imaging. (B) Plate assay as in (A). In order to assess the genetic interaction between rad55Δ and nam7Δ, a concentration of 0.008% MMS was used and plates were imaged after 3 days of growth.