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. 2016 Mar 21;44(11):5218–5230. doi: 10.1093/nar/gkw182

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — In vivo consequences of NMD on DNA damage resistance and HR. (A) Plate assay measuring the sensitivity of wild-type (WDHY2217), nam7Δ (WDHY3865) and nmd2Δ (WDHY4012) strains to MMS. Serial dilutions (1:5) of cultures were spotted onto YPD containing 0.04% MMS and grown at 30°C for 4 days prior to imaging. (B) Plot showing Ade⁺ Ura⁺ recombination rates of the indicated strains (wild-type: WDHY2371; nmd2Δ: WDHY4650; nam7Δ:WDHY4660). Rates were defined as the number of events/cell/division. Error bars represent the 95% confidence interval, non-overlapping of error bars between two samples was deemed statistically significant.