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The Rad5 interaction region is mapped to the C-terminus of Rev1 by yeast two-hybrid assays. (A) Physical interaction of Rev1 and its truncations with the full-length Rad5 or Rad5-NT164. Fragments remaining after Rev1 deletion are shown in the right panel relative to known functional domains. (B) Rev1-CT150 is the minimum region capable of interaction with Rad5. The Y2H conditions are as described in Figure 1.
