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. 2016 Apr 7;44(11):e105. doi: 10.1093/nar/gkw224

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — SiMSen-Seq. (A) Sequence composition of hairpin protected barcode primer. Different sequence elements are indicated by color. (B) Schematic design and structure of hairpin protected barcode primer. (C) Melting curve analysis of hairpin protected barcode primer using a temperature controlled spectrophotometer. The derivative of the absorption over time is shown. The melting temperature (Tm) where 50% of primers are in an open configuration is indicated. (D). Thirty-six different hairpin protected barcode primers were evaluated (Mean = 70.01, SD ± 0.24).