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. 2016 May 16;44(11):5330–5343. doi: 10.1093/nar/gkw400

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — All Drosha splice variants form the microprocessor complex with DGCR8 and bind additional interaction partners. (A) Schematic representations of the microprocessor complex (i) and an additional Drosha-containing complex (ii). A selection of interacting partners is represented. Additional proteins are indicated with dashed lines. (B) Immunoprecipitation of Flag-tagged DGCR8 pulled down its interaction partner V5-tagged Drosha. (C) Immunoprecipitation of Flag-tagged Drosha pulled down its HA-tagged microprocessor co-factor DGCR8. (D) Immunoprecipitation of YFP-tagged Drosha pulled down endogenous DGCR8, (E) endogenous EWS and p68 and (F) exogenous p68. All co-immunoprecipitation experiments were performed from cell lysates of HEK293 cells transiently transfected in at least two biological replicates with the respective Drosha splice variant (YFP-Drosha-V5) construct as well as Flag/HA-DGCR8 or Flag-AGO2 (B), HA-DGCR8 (C) or p68 (F), respectively.