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. 2016 May 31;113(24):E3461–E3467. doi: 10.1073/pnas.1603799113

Fig. S4.

Fig. S4.

Prefractionation of tryptic digests composed of cellular protein extracts from mice livers and MS-QBiC peptides using SCX chromatography. (A) A chromatogram of the tryptic digests separated with the SCX chromatography. Numbers above the chromatogram indicate numbers of the SCX fractions. (B) Examples of the SRM chromatogram. SRM chromatograms of FSQGQQLVTK* (SRM-ID 21, Upper) contained in the SCX fractions from no. 4 to no. 10 and those of YFGSIDSSENNHK* (SRM-ID 91, Lower) in the fractions from no. 11 to no. 17 are shown. (C) Distribution of the signal intensities corresponding to the target peptides originated from the MS-QBiC peptide in the SCX fractions from no. 3 to no. 18. A red color indicates that the corresponding signal was detected in the fraction. Black rows indicate that the signal was not able to be detected in any fraction.