Table S3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or property | Reference and/or source |
| E. coli | ||
| Top10 | F−, mcrA, Δ(mrr-hsdRMS-mcrBC), ϕ80, lacZΔM15, ΔlacX74, deoR, nupG, recA1, araD139, Δ(ara-leu)7697, galU, galK, rpsL(Strr), endA1 | Invitrogen |
| CWG1241 | Top10 ΔwecA::cat; Cmr | This study |
| BKT09 | Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514, ΔpagP, ΔlpxP, ΔlpxM, ΔlpxL::Kanr | (37) R. Bishop, McMaster University, Canada |
| S. enterica | ||
| H251.1 | S. enterica serovar Typhi Ty2 trp, cys, ΔaroC1019 | (38) K. E. Sanderson, Salmonella Genetic Stock Centre, University of Calgary, Canada. |
| CWG1235 | S. Typhi H251.1 ΔvexC | This study |
| CWG1236 | S. Typhi H251.1 ΔvexE | This study |
| CWG1237 | S. Typhi H251.1 ΔvexC ΔvexE | This study |
| CWG1238 | S. Typhi H251.1 ΔwaaG::kan; Kmr | This study |
| CWG1239 | CWG1236 ΔwaaG::kan; Kmr | This study |
| A. denitrificans | ||
| CWG1240 | A. denitrificans; Smr, Ampr, Kanr | G. D. Wright, McMaster University, Canada |
| Plasmids | ||
| pACYC184 | Cloning vector containing tetracycline and chloramphenicol resistance cassettes; Tetr, Cmr | (39) |
| pBAD24 | Plasmid vector with l-arabinose–inducible promoter; Apr | (40) |
| pKD3 | Source of frt-flanked chloramphenicol-resistance cassette; Cmr | (26) |
| pKD4 | Source of frt-flanked kanamycin-resistance cassette; Kmr | (26) |
| pSIM6 | λ-red recombinase helper plasmid; Apr | (27) |
| pCP20 | Source of Flp recombinase, temperature-sensitive replicon; Apr | (26) |
| pGVXN158 | pLAFR1 derivative encoding S. Typhi BRD948 viaB locus; Tetr | (11) |
| pNLP15 | spy (Spheroplast protein Y) reporter; spy promoter fused to promoterless luxCDABE operon; Kmr | (33) |
| pWQ284 | pBAD24 containing a chloramphenicol-resistance cassette; Cmr | (41) |
| pWQ782 | pACYC184 derivative containing an oligo cassette containing Asc1 and Spe1 restriction sites; Tetr | This study |
| pWQ783 | pWQ782 containing the Salmonella Typhi viaB operon; Tetr | This study |
| pWQ784 | Derived from pWQ783; viaB containing a deletion of vexE; Tetr | This study |
| pWQ785 | Derived from pWQ783; viaB containing a deletion of vexC; Tetr | This study |
| pWQ786 | pBAD24 derivative encoding Salmonella Typhi VexE-His6; Apr | This study |
| pWQ787 | pBAD24 derivative encoding A. denitrificans VexE-His6; Apr | This study |
| pWQ788 | pWQ786 containing a H487A mutation; Apr | This study |
| pWQ789 | pWQ787 containing a H466A mutation; Apr | This study |
| pWQ790 | pWQ787 containing a Y471F mutation; Apr | This study |
| pWQ791 | pBAD24 derivative encoding A. denitrificans VexL-His6; Apr | This study |
| pWQ792 | pBAD24 derivative encoding VexC-His10; Apr | This study |
| pWQ793 | pWQ284 derivative encoding VexC-His10; Cmr | This study |
| pWQ794 | pWQ284 derivative encoding A. denitrificans VexE-His6; Cmr | This study |
| pWQ795 | pWQ284 derivative encoding S. Typhi LpxL-His6; Cmr | This study |
| pWQ796 | pWQ284 derivative encoding S. Typhi LpxM; Cmr | This study |
| pWQ903 | pBAD24 derivative encoding WaaG from E. coli F470 | (42) |