Table S4.
Sequences of oligonucleotide primers used to generate recombinant plasmids, site-directed mutants, and genomic deletions
| Plasmid | Primer | Sequence (5′→3′) | Features |
| pWQ782 | SL024 | gatcggcgcgccGGTACCactagtAGCATTCATCAGGCGGGCAAG | Forward primer for amplification of pACYC184 introducing Asc1 and Spe1 restriction sites |
| SL025 | gatcggcgcgccCATCCGGAATTCCGTATGGCAATG | Reverse primer for amplification of pACYC184 introducing Asc1 and Spe1 restriction sites | |
| pWQ783 | SL035 | cattggcgcgccTCATTTCCGAAGCAGTCAC | Forward primer for amplification of viaB; Asc1 restriction site |
| MM17 | acatactagtCTTAGGCTGGGGTGTCTTTGC | Forward primer for the amplification of viaB; Asc1 restriction site. | |
| pWQ784 | SL026 | gatcactagtTTATTCACGCATCCGCCTGATG | Reverse primer for the amplification of tviABCDEvexABCD; Spe1 restriction site. |
| pWQ785 | MNL05 | AGGAGAGACGCATTCtaaTTAGTAAATATACGGATAGAGTGTGG | Forward mutagenesis primer for vexC deletion in pSDL033 |
| MNL06 | CTCTATCCGTATATTTACTAAttaGAATGCGTCTCTCCTGAAGC | Forward mutagenesis primer for vexC deletion in pSDL033 | |
| pWQ786 | AZG1 | gcgcgcgaattcaccATGAGCTTGCACTCTACATTT | Forward primer for the amplification of vexE-His6; EcoRI restriction site (S. Typhi) |
| AZG2-2 | gcgcgctctagatcagtggtggtggtggtggtgACTATCCCTACGTATAAT | Reverse primer for the amplification of vexE-His6; XbaI restriction site (S. Typhi) | |
| pWQ787 | vexeAdHis-for | tactgaattcaccATGGTTGATACGGTCATTGAAAGC | Forward primer for the amplification of vexE-His6; EcoRI restriction site (A. denitrificans) |
| vexeAdHis-rev | tacttctagattaatggtgatggtgatggtgCGCGGACGGATCCGCCGCCG | Reverse primer for the amplification of vexE-His6; EcoRI restriction site (A. denitrificans) | |
| pWQ788 | SL030 | GCATTATTGTATCGGCTgcgCTGGGCGCAATGTATGCC | Forward mutagenesis primer for H487A in VexE (S. Typhi) |
| SL031 | GGCATACATTGCGCCCAGcgcAGCCGATACAATAATGC | Reverse mutagenesis primer for H487A in VexE (S. Typhi) | |
| pWQ789 | SL032 | GGTCGCCACCGCCgccGTCGGGCCGATG | Forward mutagenesis primer for H466A in VexE (A. denitrificans) |
| SL033 | CATCGGCCCGACggcGGCGGTGGCGACC | Reverse mutagenesis primer for H466A in VexE (A. denitrificans) | |
| pWQ790 | SL063 | CACGTCGGGCCGATGtttGCGGGCCTGATGGCG | Forward mutagenesis primer for Y471F in VexE (A. denitrificans) |
| SL064 | CGCCATCAGGCCCGCaaaCATCGGCCCGACGTG | Reverse mutagenesis primer for Y471F in VexE (A. denitrificans) | |
| pWQ792 | SL083 | cagggaattcaTGTTCGGTTTATTAGGTTG | Forward primer for the amplification of vexC-His10; EcoRI restriction site (S. Typhi) |
| SL084 | gatcaagcttttaatggtggtgatggtggtgatggtggtgatgTATATCAAAGGAATAATCTTCAG | Reverse primer for the amplification of vexC-His10; HindIII restriction site (S. Typhi) | |
| pWQ791 | SL079 | cattgaattcATGCGACTTCGAAAGACAGTGATGG | Forward primer for amplification of vexL-His6; EcoRI restriction site (A. denitrificans) |
| SL081 | cattaagcttttagtggtggtggtggtggtgGCCCGGGCAACCCGCGCC | Reverse primer for amplification of vexL-His6; HindIII restriction site (A. denitrificans) | |
| pWQ795 | SL102 | cagggaattcATGACGAAGTTGCCTAAGTTC | Forward primer for amplification of LpxL-His6; EcoRI restriction site (S. Typhi) |
| SL103 | gatcaagcttttaatggtggtgatggtggtgATAGCGCGACGGTACGCCTTC | Reverse primer for amplification of LpxL-His6; HindIII restriction site (S. Typhi) | |
| pWQ796 | SL119 | caccggtacctATGGAAACCAAAAAAAATAATAGTG | Forward primer for amplification of LpxM; KpnI restriction site (S. Typhi) |
| SL120 | gatcaagcttTTATTTGATGGGATAAAGATC | Reverse primer for amplification of LpxM; HindIII restriction site (S. Typhi) | |
| CWG1236 | SL089 | CCTGATAACCATCAGGCGGATGCGTGAATAAGGCTGAGTAAGGAAATATAgtgtaggctggagctgcttc | λ-red recombination primer for vexE deletion (S. Typhi) |
| SL090 | ATTTTCAGCTCTGAAGTACAAATTTTCATCTACCGCAATTAAATCGCTTAcatatgaatatcctccttag | λ-red recombination primer for vexE deletion (S. Typhi) | |
| CWG1235, CWG1237 | SL092 | GATTTGTGACACTGAAAATCACTGACTTTAGCTTCAGGAGAGACGCATTCgtgtaggctggagctgcttc | λ-red recombination primer for vexC deletion (S. Typhi) |
| SL093 | CATTTTTTAATACGTTCTGAATTTTCCACACTCTATCCGTATATTTACTAAcatatgaatatcctccttag | λ-red recombination primer for vexC deletion (S. Typhi) | |
| CWG1239, CWG1240 | SL096 | GAAAAAATGCTGCCGCATGAGGCACGCCCCATAGATTTGGACAGCCTGCTgtgtaggctggagctgcttc | λ-red recombination primer for waaG deletion (S. Typhi) |
| SL097 | CCTCAAAAGCATCTTTACCGCGCCATAGTGTGGTTAACGGCGCTTTCAGCcatatgaatatcctccttag | λ-red recombination primer for waaG deletion (S. Typhi) | |
| CWG1241 | LRWecA-f | GGTCTTCGTGGTTATACTTCTGCTAATAATTTTCTCTGAGAGCATGCATTgtgtaggctggagctgcttc | λ-red recombination primer for wecA deletion (E. coli) |
| LRWecA-r | AGCGTCTTCGGCCGGTTTCCCAGGCATTGGTTGTGTCATCACATCCTCATcatatgaatatcctccttag | λ-red recombination primer for wecA deletion (E. coli) |
Restriction sites are underlined. Nonchromosomal sequences are lowercase. Point mutations are boldface.