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. 2016 May 31;113(24):E3349–E3358. doi: 10.1073/pnas.1523810113

Fig. 2.

Fig. 2.

Focal ROS production induces translocation of LC3 and autophagy receptors to damaged mitochondria. (Left) Maximum intensity projections of HeLa cells expressing Parkin, Mito-SNAP (blue), GFP-LC3 (green), and MitoKR (red), as well as Halo-OPTN (A), Halo-NDP52 (B), or Halo-TAX1BP1 (C, magenta) 90 min after activation of MitoKR by 561-nm laser light. (A) At 90 min postbleaching, OPTN and LC3 selectively translocate to fragmented mitochondria (arrows) within the bleach window, but not to unbleached mitochondria on the opposite side of the cell. (B) At 90 min post-MitoKR activation, NDP52 and LC3 are robustly recruited to bleached mitochondria (arrows). (C) TAX1BP1 and LC3 are recruited to damaged mitochondria within the bleach windows 90 min after MitoKR activation (arrows). [Scale bars: A–C (full size), 10 μm; A–C (zoom), 2.5 μm.]