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. 2016 May 31;113(24):E3349–E3358. doi: 10.1073/pnas.1523810113

Fig. 5.

Fig. 5.

OPTN depletion blocks efficient mitophagy. (A) Representative confocal images of mitochondria-positive autophagosomes at 180 min post-CCCP in Parkin-expressing cells. [Scale bars: A (full size), 10 μm; A (zoom), 2.5 μm.] (B) Depletion of OPTN significantly impairs autophagosome formation at 180 min post-CCCP. In contrast, knockdown of NDP52 does not affect LC3 recruitment to depolarized mitochondria at this time point. Data were collected from 29 to 49 cells from at least three independent experiments. Bars represent mean ± SEM. ***P < 0.001. n.s., not significant.