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. 2016 May 31;113(24):E3349–E3358. doi: 10.1073/pnas.1523810113

Fig. S2.

Fig. S2.

(A) Halo-TAX1BP1, mCherry-OPTN, and GFP-NDP52 colocalize on depolarized mitochondria 90 min post-CCCP in Parkin-expressing HeLa cells. (B and C) Recruitment of either NDP52 or TAX1BP1 to depolarized mitochondria is dependent on Parkin and blocked by expression of the Parkinson’s disease-associated Parkin-T240R mutant. HeLa cells transfected with Mito-DsRed2, mCherry-Parkin-T240R, and either Halo-NDP52 (B) or Halo-TAX1BP1 (C). At 60 min post-CCCP, Parkin-T240R is recruited to depolarized mitochondria, but NDP52 and TAX1BP1 remain largely cytosolic. (D) In the absence of exogenous Parkin, NDP52 and LC3 fail to translocate to depolarized mitochondria at 90 min post-CCCP. [Scale bars: A, 5 μm; B–D (full size), 10 μm; B–D (zoom), 2.5 μm.]