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(A) Mitochondria double-labeled with Mito-SNAP (blue) and the photosensitizer MitoKR (red). Before bleaching (Top), Mito-SNAP and MitoKR signals appear identical. After sustained laser illumination with 561-nm light (Bottom), the MitoKR signal disappears but Mito-SNAP still clearly labels mitochondria. (B) Confocal time series showing recruitment of YFP-Parkin within 12–15 min of MitoKR photobleaching. (Scale bars: 10 μm.)
