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. 2016 May 31;113(24):6677–6682. doi: 10.1073/pnas.1602875113

Fig. 5.

Fig. 5.

Eqt-SM and GPI are cosorted into vesicles that bud from the Golgi. (A) Time-lapse galleries showing example Golgi budding events. The fluorescent proteins (mKate2-FM4-GPI or mKate2-FM4-CD8α) were released from the ER of HeLa cells stably expressing Eqt-SM-oxGFP, and image acquisition of the Golgi region was initiated 30 min later. An example of a vesicle containing both Eqt-SM and GPI is shown at top, and an example of vesicle that contains CD8α, but not Eqt-SM, is shown below. (B) Time-lapse gallery of the Golgi region of a HeLa cell expressing SBP-mKate2-GPI and eGFP-FM4-CD8α, showing examples of each reporter protein being exported from the Golgi in distinct carriers. (C) Summary of cargo loads of vesicles that bud from the Golgi. The means ± SDs of Eqt-SM-oxGFP budding events in cells expressing mKate2-FM4-GPI (n = 33), mKate2-FM4-CD8α (n = 26), and SBP-mKate2-GPI and eGFP-FM4-CD8α (n = 69) are shown.