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. 2016 May 31;113(24):6677–6682. doi: 10.1073/pnas.1602875113

Fig. S3.

Fig. S3.

Inhibition of SM synthesis does not ablate the Golgi pool of DAG. DAG was detected in the Golgi of SMS1 and SMS2 RNAi cells (A) and in the Golgi of cells treated with D609 (B). Cells were transfected with a plasmid that directs the expression of a GST-tagged C2 domain of protein kinase D, a DAG biosensor (37), which was detected by anti-GST immunofluorescence. The proportions of PKD_C2 fluorescence that localize to the Golgi are indicated in each micrograph (mean ± SD; n > 20). (Scale bars: 10 μm.)