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Quantitation of intracellular vesicles containing Eqt-SM
| Condition | Vesicles/cell |
| Eqt-SM | 138 ± 47 |
| Eqt-sol* | 18 ± 10 |
| 20 °C, 1 h | 12 ± 7 |
| 20 °C, 1 h -> 37 °C, 30 min | 42 ± 22 |
| siRNA – control | 80 ± 22 |
| siRNA – SMS1+SMS2 | 22 ± 11 |
| Vehicle control | 65 ± 14 |
| D609 (200 μM, 3 h) | 14 ± 8 |
Eqt-sol containing vesicles were quantified.
The mean number of vesicles outside the Golgi region of each cell cytoplasm (n >20 cells for each condition) was quantified using the ImageJ Analyze Particles plug-in.
