Table S1.
Statistics of crystallographic analyses
| pSTING/IRF-3 | pMAVS/IRF-3 | pTRIF/IRF-3 | IRF-3/CBP | pNSP1/IRF-3 | NSP1/IRF-3 | |
| Data collection | ||||||
| Space group | P2 | P21 | P3221 | P21 | P3221 | P212121 |
| Cell dimensions | ||||||
| a, b, c (Å) | 60.08, 56.40, 75.55 | 38.80, 104.91, 66.27 | 72.81, 72.81, 127.60 | 85.23, 88.66, 92.81 | 72.98, 72.98, 127.55 | 67.77, 107.92, 135.65 |
| α, β, γ (°) | 90.0, 104.64, 90.0 | 90.0, 106.13, 90.0 | 90.0, 90.0, 120.0 | 90.0, 113.09, 90.0 | 90.0, 90.0, 120.0 | 90.0, 90.0, 90.0 |
| Resolution, Å | 2.00 (2.03–2.00) | 2.40 (2.49–2.40) | 1.60 (1.63–1.60) | 2.50 (2.59–2.50) | 1.72 (1.75 to1.72) | 2.90 (2.95–2.90) |
| Unique reflections | 35,085 | 19,995 | 52,282 | 42,426 | 42,593 | 22,409 |
| Molecules per ASU | 2 | 2 | 1 | 4 | 1 | 4 |
| Rmerge (%) | 10.8 (57.0) | 9.9 (136) | 5.6 (>100) | 13.8 (155) | 6.8 (>100) | 14.0 (89.8) |
| Rp.i.m. (%) | 6.6 (35.0) | 4.0 (53.9) | 1.7 (33.4) | 8.5 (94.6) | 2.2 (39.8) | 5.6 (35.6) |
| CC1/2 | (0.817) | (0.697) | (0.818) | (0.384) | (0.823) | (0.781) |
| I/σI | 14.0 (1.8) | 14.3 (1.7) | 45.2 (1.8) | 6.2 (0.8) | 44.5 (1.9) | 15.7 (2.1) |
| Completeness (%) | 98.2 (83.7) | 100 (100) | 99.8 (99.5) | 96.3 (85.9) | 100 (100) | 100 (99.9) |
| Redundancy | 3.6 (3.2) | 7.2 (7.3) | 11.9 (11.6) | 3.5 (3.4) | 11.8 (10.9) | 7.1 (7.3) |
| Refinement | ||||||
| Resolution (Å) | 2.00 | 2.40 | 1.60 | 2.50 | 1.72 | 2.90 |
| No. reflections used | 35,068 | 19,966 | 52,240 | 42,378 | 42,537 | 22,342 |
| Rwork/Rfree (%) | 17.6/23.7 | 18.8/25.1 | 16.8/18.7 | 19.2/24.2 | 17.4/19.0 | 21.0/25.0 |
| No. atoms | 4,341 | 3,968 | 2,228 | 7,876 | 2,207 | 7,718 |
| Protein | 3,669 | 3,678 | 1,851 | 7,650 | 1,885 | 7,386 |
| Ligand/ion | 292 | 220 | 110 | N.A. | 102 | 332 |
| Water | 380 | 70 | 267 | 226 | 220 | 0 |
| B-factors | ||||||
| Protein | 33.6 | 64.9 | 32.7 | 62.8 | 39.4 | 46.3 |
| Ligand/ion | 58.9 | 113.2 | 39.3 | N.A. | 60.4 | 63.9 |
| Water | 37.5 | 52.9 | 42.2 | 52.3 | 46.3 | N.A. |
| Rmsd | ||||||
| Bond lengths (Å) | 0.007 | 0.002 | 0.006 | 0.003 | 0.007 | 0.002 |
| Bond angles (°) | 1.004 | 0.605 | 1.039 | 0.624 | 0.999 | 0.589 |
| PDB ID code | 5JEJ | 5JEK | 5JEL | 5JEM | 5JEO | 5JER |
One crystal was used to collect each of the datasets. Values in parentheses are for the highest-resolution shells.